John Kilduff, the show's host, is an exhibited artist and longtime Los Angeles resident. He received comedic training at The Groundlings, studied improv at Los Angeles City College, and attended the Otis/Parsons Art Institute.In 2008 he received a MFA at UCLA in California.

Kilduff has appeared as a guest on The Tyra Banks Show, where he painted Banks' portrait. He appeared on the red carpet for the VH1 2006 Big Awards, and painted David Hasselhoff's portrait on the second season of America's Got Talent. Kilduff was also filmed painting and jogging for a 2006 USA Network commercial.

LA Weekly notes that during most episodes of Let's Paint TV, Kilduff wears "his trademark crumpled and paint-stained Brooks Brothers suit." In order to avoid working a day job, Kilduff sells many of his paintings at art fairs around California. He has appeared at several art shows throughout Southern California, often running on his treadmill, painting, and doing various other activities while museum patrons look on.